Coding

Part:BBa_K5250000:Design

Designed by: Ekaterina Tocheva, Seraina Mueller, Vanessa Doerr   Group: iGEM24_UZurich   (2024-09-28)


XutR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 53
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 622
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been codon optimized.

Source

The part originates from Pseudomonas fluorescens. Sequence found in the following paper: https://doi.org/10.1128/aem.00250-20

References

Callaghan, J. D., Stella, N. A., Lehner, K. M., Treat, B. R., Brothers, K. M., St Leger, A. J., & Shanks, R. M. Q. (2020). Xylose-Inducible promoter tools for pseudomonas species And their use in implicating a role for the type II secretion system protein XCPQ in the inhibition of corneal epithelial wound closure. Applied and Environmental Microbiology, 86(14). https://doi.org/10.1128/aem.00250-20